Journal: Viruses

Article Title: Whole-Genome Sequencing of Human Enteroviruses from Clinical Samples by Nanopore Direct RNA Sequencing

doi: 10.3390/v12080841

Figure Lengend Snippet: Taxonomic classification of viral reads (Oxford Nanopore) and contigs (Illumina MiSeq) using MEGAN based on BLASTN matches against the NCBI’s nucleotide ( nt ) database at the species and genotype levels. Asterisks (*) indicate that >90% of the respective genome sequence was covered, while bold numbers indicate EV species identity confirmed by Sanger sequencing of the VP1 gene. Minus sign (−) indicates absence of hits in the dataset.

Article Snippet: Sequence identity between DRS and Illumina MiSeq enterovirus consensus sequences ranged between 94% and 97%.

Techniques: Sequencing, Virus

Journal: The American Journal of Pathology

Article Title: Long Interspersed Element-1 Protein Expression Is a Hallmark of Many Human Cancers

doi: 10.1016/j.ajpath.2014.01.007

Figure Lengend Snippet: LINE-1–encoded protein ORF1p is undetectable in mature human somatic tissues and commonly expressed in a wide range of human cancers. Representative photomicrographs are shown in pairs, with normal tissues on the left; tissues are counterstained (blue). Photomicrographs on the right show LINE-1 ORF1p immunoreactivity (brown) detected with a rabbit polyclonal ORF1p antibody in various human neoplasms, including colon carcinoma (A), renal cell carcinoma (B), lymphoma (C), hepatocellular carcinoma (D), lung carcinoma (E), breast carcinoma (F), pancreatic carcinoma (G), and biliary tract carcinoma (H). Original magnification, ×160 (A–H). Scale bar = 20 μm. N, normal tissue; T, tumor.

Article Snippet: Monoclonal Anti-Human ORF1p Antibody Fifteen peptides from the LINE-1 ORF1p consensus sequence ( {"type":"entrez-protein","attrs":{"text":"AAB60344.1","term_id":"483915","term_text":"AAB60344.1"}} AAB60344.1 , 338 amino acids) were selected as potential surface epitopes antibody library antigens by Abmart, Inc (Shanghai, China).

Techniques:

Journal: The American Journal of Pathology

Article Title: Long Interspersed Element-1 Protein Expression Is a Hallmark of Many Human Cancers

doi: 10.1016/j.ajpath.2014.01.007

Figure Lengend Snippet: LINE-1 ORF1p is present in a wide range of human neoplasms. A: LINE-1 expression as a percentage of LINE-1–immunoreactive cases in different primary tumor types. LINE-1 immunolabeling in low- versus high-grade mesenchymal tumors (B), lymphomas (C), pancreatic ductal neoplasms (D), and astrocytic neoplasms (E), expressed as a percentage of cases. The number above each column in panels B–E designates the number of cases examined for LINE-1 immunolabeling.

Article Snippet: Monoclonal Anti-Human ORF1p Antibody Fifteen peptides from the LINE-1 ORF1p consensus sequence ( {"type":"entrez-protein","attrs":{"text":"AAB60344.1","term_id":"483915","term_text":"AAB60344.1"}} AAB60344.1 , 338 amino acids) were selected as potential surface epitopes antibody library antigens by Abmart, Inc (Shanghai, China).

Techniques: Expressing, Immunolabeling

Journal: PLoS Genetics

Article Title: Single-molecule dynamics and genome-wide transcriptomics reveal that NF-kB (p65)-DNA binding times can be decoupled from transcriptional activation

doi: 10.1371/journal.pgen.1007891

Figure Lengend Snippet: (A) p65 forms a heterodimeric complex with p50 in the cytosol (cyt). The complex is bound by the cytosolic inhibitor IκBa that prevents its translocation into the nucleus. Following TNF-α stimulation and IκBα dissociation, p65/p50 translocate into the nucleus allowing subsequent DNA binding and gene activation. (B) We used a copy of the human p65 fused to a Halo tag as a basis for our mutational expression system. P65-Halo constructs were then expressed in HeLa cells and fluorescently labelled with a JF549 Halo ligand. Upon TNF-α stimulation, labelled p65-Halo translocates into the nucleus. Scale bar: 10 μm.

Article Snippet: Synthetic HPLC-purified sense and anti-sense oligo probes encoding the consensus binding sequence of p65 were purchased from Microsynth (Microsynth AG, Switzerland; Sense-p65_κB: 5’-AGTTGAG GGGACTTTCC CAGGC-3’; Anti-sense-p65_κB: 5’-GCCTG GGAAAGTCCC CTCAACT-3’).

Techniques: Translocation Assay, Binding Assay, Activation Assay, Expressing, Construct

Journal: PLoS Genetics

Article Title: Single-molecule dynamics and genome-wide transcriptomics reveal that NF-kB (p65)-DNA binding times can be decoupled from transcriptional activation

doi: 10.1371/journal.pgen.1007891

Figure Lengend Snippet: (A) Single particle tracking (SPT) was performed after TNF-α stimulation and p65-Halo translocation into the nucleus. All recorded trajectories were filtered based on a spatial threshold established using an immobile control (Histone subunit H2B, see ). After filtering out mobile molecules (i) and correction of photobleaching, the DNA binding time could be estimated from the length of each individual trajectory. ( B ) Schematic overview of p65 affinity mutants. (C) Normalized survival probability plots (1-CDF plot) of the DNA-bound fraction for p65-WT as well as the DNA affinity mutants KKAA and KKRR. The distributions were fitted using a bi-exponential function revealing the fast (t b fast) and slow (t b slow) DNA binding times. (D) Summary of the obtained fitting parameters together with the relative DNA dissociation constant K D for each construct. The pie chart shows the fraction of events associated to the fast (grey) or slow binding time. K on * was obtained from single step displacement histograms as described in Methods. While all constructs exhibit similar k on * as well as t b fast, the slow binding time t b slow correlates with the DNA affinity.

Article Snippet: Synthetic HPLC-purified sense and anti-sense oligo probes encoding the consensus binding sequence of p65 were purchased from Microsynth (Microsynth AG, Switzerland; Sense-p65_κB: 5’-AGTTGAG GGGACTTTCC CAGGC-3’; Anti-sense-p65_κB: 5’-GCCTG GGAAAGTCCC CTCAACT-3’).

Techniques: Single-particle Tracking, Translocation Assay, Control, Binding Assay, Construct

Journal: PLoS Genetics

Article Title: Single-molecule dynamics and genome-wide transcriptomics reveal that NF-kB (p65)-DNA binding times can be decoupled from transcriptional activation

doi: 10.1371/journal.pgen.1007891

Figure Lengend Snippet: (A) We assessed the level of gene activation using RNA sequencing (RNA-Seq). To this end, total mRNA was isolated and sequenced using next-generation sequencing. The total initial set of 1080 genes was cross-referenced using the Chip-Seq ENCODE database, providing a subset of 215 direct interacting genes. A second subset of 45 genes consist of known NFκB regulated genes. For each p65 mutant, the fold-change (FC) expression above the non-transfected (NT) control was calculated and for each gene compared with p65-WT using a log-log FC plot. As a general discrimination between up- and downregulated genes compared to p65-WT, we calculated the logFC ratio. Values with logFC ratio>1 are marked upregulated (red), those with logFC ratio<1 are marked downregulated (green). (B) FC values were standardized (per gene) and the different conditions clustered hierarchically to identify similarities. Interestingly, p65-WT co-clusters with p65-KKRR, which also shows the highest average z-score ( B , top plot) and was identified as the only gain-of-function mutant (C) . The two transactivation mutants as well as the low affinity mutant and p65-ΔDNA also co-cluster highlighting their functional similarity. (C) Classification of each p65 variant based on the logFC ratio estimator, showing that p65-KKRR (i.e. with higher DNA affinity) represents the only gain-of-function mutant. (D) RNA-Seq analysis comparing transcriptional activation of p65-KKAA and p65-KKRR with p65-WT. p65-KKAA shows very weak correlation with p65-WT as well as a strongly reduced gene activation (logFC ratio = -0.47) indicating a loss of gene specificity as well as activation potential. In contrast, p65-KKRR shows higher correlation as well as an increased gene activation (logFC ratio = 0.28).

Article Snippet: Synthetic HPLC-purified sense and anti-sense oligo probes encoding the consensus binding sequence of p65 were purchased from Microsynth (Microsynth AG, Switzerland; Sense-p65_κB: 5’-AGTTGAG GGGACTTTCC CAGGC-3’; Anti-sense-p65_κB: 5’-GCCTG GGAAAGTCCC CTCAACT-3’).

Techniques: Activation Assay, RNA Sequencing, Isolation, Next-Generation Sequencing, ChIP-sequencing, Mutagenesis, Expressing, Transfection, Control, Functional Assay, Variant Assay

Journal: PLoS Genetics

Article Title: Single-molecule dynamics and genome-wide transcriptomics reveal that NF-kB (p65)-DNA binding times can be decoupled from transcriptional activation

doi: 10.1371/journal.pgen.1007891

Figure Lengend Snippet: ( A ) Schematic overview of p65 truncation mutants. ( B ) Normalized survival probability (1-CDF plot) plots of the DNA-bound fraction for p65-WT as well as the transactivation mutants ΔTA1 and ΔTAD as well as a mutant with removed DNA-binding domain (ΔDNA). The distributions were fitted using a bi-exponential function revealing the fast (t b fast) and slow (t b slow) DNA binding times. ( C ) As for the DNA affinity mutants, we found k on * to be in a similar range for all the tested constructs. ( D ) RNA-Seq analysis revealed very low residual transcriptional activation of p65-ΔDNA as evident by logFC ratio = -0.45. The two transactivation mutants showed good correlation with p65-WT (r ~ 0.8) but at strongly reduced transcript abundance resulting in logFC ratio around -0.25.

Article Snippet: Synthetic HPLC-purified sense and anti-sense oligo probes encoding the consensus binding sequence of p65 were purchased from Microsynth (Microsynth AG, Switzerland; Sense-p65_κB: 5’-AGTTGAG GGGACTTTCC CAGGC-3’; Anti-sense-p65_κB: 5’-GCCTG GGAAAGTCCC CTCAACT-3’).

Techniques: Mutagenesis, Binding Assay, Construct, RNA Sequencing, Activation Assay

Journal: PLoS Genetics

Article Title: Single-molecule dynamics and genome-wide transcriptomics reveal that NF-kB (p65)-DNA binding times can be decoupled from transcriptional activation

doi: 10.1371/journal.pgen.1007891

Figure Lengend Snippet: ( A ) The median log 2 FC ratio as retrieved from RNA-Seq data is plotted against t b slow . Note that ΔDNA affinity has been assigned to an arbitrarily low value. ( B ) Working model for p65 mediated transcriptional activation. (1) P65 can act as a pioneering TF, open the chromatin and bind its consensus DNA sequence. Transcriptional activation can then be initiated although the exact mechanism of RNA pol-II recruitment remains unclear. Following this model, the DNA binding time would correlate with the transcriptional output, while removal of TADs would not affect the complex stability. (2) An important extension of this model as suggested by our data is that TADs are required to efficiently translate p65 DNA binding into transcriptional output presumably through the recruitment of protein co-factors.

Article Snippet: Synthetic HPLC-purified sense and anti-sense oligo probes encoding the consensus binding sequence of p65 were purchased from Microsynth (Microsynth AG, Switzerland; Sense-p65_κB: 5’-AGTTGAG GGGACTTTCC CAGGC-3’; Anti-sense-p65_κB: 5’-GCCTG GGAAAGTCCC CTCAACT-3’).

Techniques: RNA Sequencing, Activation Assay, Sequencing, Binding Assay

Journal: Genome Research

Article Title: The mutational spectrum of non-CpG DNA varies with CpG content

doi: 10.1101/gr.103283.109

Figure Lengend Snippet: Primate L1 families. A full-length generic primate L1 element is shown. The primate-specific L1Pa families examined here are placed on a simplified primate tree according to their average ages (see Methods). The columns give the KB of ORFII orthologs of the various families used for the various analyses in this paper (see Methods). M, P, and H indicate Macaca mulatta (macaque, Old World monkey), Pan troglodytes (chimpanzee), and Homo sapiens (human), respectively. The ages for the divergences of theses species were derived as described earlier (Walser et al. 2008). See Methods and Results, for details on the various steps outlined on the right side of the figure.

Article Snippet: Consensus sequences of L1 families These were derived as described earlier ( Walser et al. 2008 ) except we limited ourselves to the ∼3800 bp (depending on the family) ORF2 sequence because we could use the highly conserved amino acid sequence of the ORF2 protein as a guide for aligning the base sequences.

Techniques: Derivative Assay

Journal: Genome Research

Article Title: The mutational spectrum of non-CpG DNA varies with CpG content

doi: 10.1101/gr.103283.109

Figure Lengend Snippet: Mutational fate of each of the four bases for different L1 families. Panel A shows the distribution of A mutations to G, C, and T that occurred between the human and chimpanzee lineages for each L1Pa family. There are two x-axes on the bottom of panel A: the top one (boxed in gray) gives the total mutations (N × 103) that occurred and the bottom one (in bold) indicates the L1Pa family. For example, we found (Methods, Determination of the Mutational Spectrum) that 17,458 substitutions of A occurred between the chimpanzee and human L1Pa3 orthologs (rounded to 17.5 × 103 in Fig. 5): 8387 and 9071, respectively, for the human and chimpanzee orthologs. Of the total, 10,095 (0.58) were G transitions (green bar, left y-axis), 4595 (0.26) were C transversions (red bar, right y-axis), and 2768 (0.16) were T transversions (blue bar, right y-axis). Note that the left (transitions) and right (transversions) axes cover different ranges. The numbers of A mutations to G, and A transversions to C or T were about the same for chimpanzee and human (results not shown). For L1Pa4, 16,678 (16.7 × 103) A mutations occurred, again with about one-half occurring in chimpanzee and human, and again the numbers of G transitions and C or T transversions were about the same in chimpanzees and human. And so on for the rest of the families in panel A and for the mutations of G, C, and T presented in panels G, C, and T respectively. In each case the green bar (left axis) shows transitions and the red and blue bars (right axis) show transversions. The gray line is the total non-CpG divergence for each L1Pa family normalized to that of L1Pa3, set to 1.0. Families that differ in total non-CpG divergence generally differ in their proportions of transitions and transversions, especially in regard to mutations of A, G, and C (much less so for T). For example, chi-square comparisons in panel A showed that the proportion of transitions and transversions in L1Pa3 are significantly different from that of L1Pa4 (indicated by the asterisks between these families). Likewise the distribution of transitions and transversions in L1Pa4 is significantly different from that of L1Pa5, but this is not the case for proportions of transitions and transversion between L1Pa7 and L1Pa8.

Article Snippet: Consensus sequences of L1 families These were derived as described earlier ( Walser et al. 2008 ) except we limited ourselves to the ∼3800 bp (depending on the family) ORF2 sequence because we could use the highly conserved amino acid sequence of the ORF2 protein as a guide for aligning the base sequences.

Techniques: